Coelacanth genome surfaces

USF part of interntional project yielding unexpected insights from a fish with a 300-million-year-old fossil record

April 17, 2013  – Researchers from the University of South Florida were members of an international team that has decoded the genome of a creature whose evolutionary history is both enigmatic and illuminating: the African coelacanth.  A sea-cave dwelling, five-foot long fish with limb-like fins, the coelacanth was once thought to be extinct.

A living coelacanth was discovered off the African coast in 1938, and since then, questions about these ancient-looking fish – popularly known as “living fossils” – have loomed large. Coelacanths today closely resemble the fossilized skeletons of their more than 300-million-year-old ancestors. Its genome confirms what many researchers had long suspected: genes in coelacanths are evolving more slowly than in other organisms.

“It has been a highly rewarding experience to be involved in another project of this scope, with such a distinguished international team of scientists,” said Gary Litman, PhD, a USF Distinguished University Professor and Hines Professor of Pediatrics at USF Health.   “As we continue to expand our understanding of genomes, it never ceases to amaze us how many different (evolutionary) solutions there have been for creating a functional immune system.”

Dr. Litman, an international expert in the field of immunogenetics, directs the molecular genetics laboratory at the St. Petersburg-based USF Children’s Research Institute.   He and colleagues John Cannon, PhD, assistant professor of pediatrics at USF, and Gail Mueller,  laboratory coordinator-All Children’s Hospital, are among the authors of a paper on the coelacanth genome, which appears in the journal Nature this week.

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Coelacanths can be huge, reaching 5 feet or more and weighing 198 pounds.

Using fish as a good model for human immunity, Dr. Litman’s research has led to the discovery of many novel genes that offer insight into how the immune system develops in children.  He was also a member of the team to first sequence all the gene receptors tht appear to play a critical role in the innate immune protection of zebrafish.  His papers have appeared in other such distinguished journals as Proceedings of the National Academy of Sciences,  Nature Immunology and Science.

In the coelacanth genome project,  the researchers  found that the genes overall were evolving significantly slower than in every other fish and land vertebrate they examined.

“This is the first time that we’ve had a big enough gene set to really see that.”said Jessica Alföldi, a research scientist at the Broad Institute and co-first author of  the Nature paper

Researchers hypothesize that this slow rate of change may be because coelacanths simply have not needed to change: they live primarily off of the Eastern African coast (a second coelacanth species lives off the coast of Indonesia), at ocean depths where relatively little has changed over the millennia.

“We often talk about how species have changed over time,” said Kerstin Lindblad-Toh, scientific director of the Broad Institute’s vertebrate genome biology group and senior author. “But there are still a few places on Earth where organisms don’t have to change, and this is one of them. Coelacanths are likely very specialized to such a specific, non-changing, extreme environment – it is ideally suited to the deep sea just the way it is.”

Because of their resemblance to fossils dating back millions of years, coelacanths today are often referred to as “living fossils” – a term coined by Charles Darwin. But the coelacanth is not a relic of the past brought back to life: it is a species that has survived, reproduced, but changed very little in appearance for millions of years.

“It’s not a living fossil; it’s a living organism,” said Alföldi. “It doesn’t live in a time bubble; it lives in our world, which is why it’s so fascinating to find out that its genes are evolving more slowly than ours.”

The coelacanth genome has also allowed scientists to test other long-debated questions. For example, coelacanths possess some features that look oddly similar to those seen only in animals that dwell on land, including “lobed” fins, which resemble the limbs of four-legged land animals (known as tetrapods). Another odd-looking group of fish known as lungfish possesses lobed fins too. It is likely that one of the ancestral lobed-finned fish species gave rise to the first four-legged amphibious creatures to climb out of the water and up on to land, but until now, researchers could not determine which of the two is the more likely candidate.

In addition to sequencing the full genome – nearly 3 billion “letters” of DNA – from the coelacanth, the researchers also looked at RNA content from coelacanth (both the African and Indonesian species) and from the lungfish. This information allowed them to compare genes in use in the brain, kidneys, liver, spleen and gut of lungfish with gene sets from coelacanth and 20 other vertebrate species. Their results suggested that tetrapods are more closely related to lungfish than to the coelacanth.

However, the coelacanth is still a critical organism to study in order to understand what is often called the water-to-land transition. Lungfish may be more closely related to land animals, but its genome remains inscrutable: at 100 billion letters in length, the lungfish genome is simply too unwieldy for scientists to sequence, assemble, and analyze. The coelacanth’s more modest-sized genome (comparable in length to our own) is yielding valuable clues about the genetic changes that may have allowed tetrapods to flourish on land.

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Gary Litman, PhD, one of the world’s top experts in immunogenetics, leads the molecular genetics laboratory at the USF Children’s Research Institute.

By looking at what genes were lost when vertebrates came on land as well as what regulatory elements – parts of the genome that govern where, when, and to what degree genes are active – were gained, the researchers made several unusual discoveries:

 -  Sense of smell. The team found that many regulatory changes influenced genes involved in smell perception and detecting airborne odors. They hypothesize that as creatures moved from sea to land, they needed new means of detecting chemicals in the environment around them.

- Immunity. The researchers found a significant number of immune-related regulatory changes when they compared the coelacanth genome to the genomes of animals on land. They hypothesized that these changes may be part of a response to new pathogens encountered on land.

- Evolutionary development. Researchers found several key genetic regions that may have been “evolutionarily recruited” to form tetrapod innovations such as limbs, fingers and toes, and the mammalian placenta. One of these regions, known as HoxD, harbors a particular sequence that is shared across coelacanths and tetrapods. It is likely that this sequence from the coelacanth was co-opted by tetrapods to help form hands and feet.

- Urea cycle. Fish get rid of nitrogen by excreting ammonia into the water, but humans and other land animals quickly convert ammonia into less toxic urea using the urea cycle. Researchers found that the most important gene involved in this cycle has been modified in tetrapods.

Sequencing the full coelacanth genome was uniquely challenging for many reasons. Coelacanths are an endangered species, meaning that samples available for research are almost nonexistent. This meant that each sample obtained was precious: researchers would have “one shot” at sequencing the collected genetic material, according to Alföldi. But the difficulties in obtaining a sample and the technical challenges of sequencing it also knit the community together.

Researchers from 40 institutions across 12 countries contributed to this work. Many funding agencies around the world provided support, including the African Coelacanth Ecosystem Programme of the South African National Department of Science and Technology, which supported the collection of samples, and the National Human Genome Research Institute, which supported the Broad Institute’s contributions including genome sequencing.

Although its genome offers some tantalizing answers, the research team anticipates that further study of the fish’s immunity, respiration, physiology, and more will lead to deep insights into how some vertebrates adapted to life on land, while others remained creatures of the sea.