synapses Archives - USF Health News https://hscweb3.hsc.usf.edu/blog/tag/synapses/ USF Health News Mon, 12 Jul 2021 21:55:40 +0000 en-US hourly 1 https://wordpress.org/?v=6.5.3 BIN1 deficit impairs brain cell communication, memory consolidation https://hscweb3.hsc.usf.edu/blog/2020/03/11/bin1-deficit-impairs-brain-cell-communication-memory-consolidation/ Wed, 11 Mar 2020 14:58:15 +0000 https://hscweb3.hsc.usf.edu/?p=31011 Preclinical study by a University of South Florida Health-University of Chicago research team offers new insights into how neuronal protein BIN1 may boost Alzheimer’s disease risk TAMPA, Fla […]

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Preclinical study by a University of South Florida Health-University of Chicago research team offers new insights into how neuronal protein BIN1 may boost Alzheimer’s disease risk

TAMPA, Fla (March 11, 2020) — Bridging integrator 1, known as BIN1, is the second most common risk factor for late-onset Alzheimer’s disease, according to genome-wide studies of genetic variants. Yet, scientists know little about what this protein does in the brain.

Now a new preclinical study has discovered that a lack of BIN1 leads to a defect in the transmission of neurotransmitters that activate the brain cell communication allowing us to think, remember and behave. Led by Gopal Thinakaran, PhD, of the University of South Florida Health (USF Health) Morsani College of Medicine and colleagues at the University of Chicago, the study was published March 10 in Cell Reports.

USF Health’s Gopal Thinakaran, PhD, leads one of the few groups around the country studying BIN1 as a risk factor for late-onset Alzheimer’s disease. | Photo by Freddie Coleman

Approximately 40% of people with Alzheimer’s disease have one of three variations in the BIN1 gene – a glitch in a single DNA building block (nucleotide) that heightens their risk for the neurodegenerative disease, said the paper’s senior author Dr. Thinakaran, a professor of molecular medicine at the USF Health Byrd Alzheimer’s Center and associate dean for neuroscience research at the Morsani College of Medicine.

“Our findings that BIN1 localizes right at the point of presynaptic communication and may be precisely regulating neurotransmitter vesicle release brings us much closer to understanding how BIN1 could exert its function as a common risk factor for Alzheimer’s disease,” Dr. Thinakaran said. “We suspect it helps control how efficiently neurons communicate and may have a profound impact on memory consolidation – the process that transforms recent learned experiences into long-term memory.”

The research team created a mouse model in which the BIN1 gene was selectively inactivated, or knocked out, to characterize the protein’s normal function in the brain. In particular, they used advanced cell and molecular biology techniques to investigate the role of BIN1 in regulating synapses associated with learning and memory.

Peering into the brain, one synapse at a time: Electron micrograph shows selected region of a mouse brain hippocampus, the brain area responsible for learning and memory. A single synapse is marked with the yellow outline. | Image courtesy of Gopal Thinakaran, USF Health Morsani College of Medicine

To frame the study results, it helps to know that a healthy human brain contains tens of billions of brain cells (neurons) that process and transmit chemical messages (neurotransmitters) across a tiny gap between neurons called a synapse. In the Alzheimer’s disease brain, this synaptic communication is destroyed, progressively killing neurons and ultimately causing a steep decline in memory as well as other signs of dementia. Individuals most susceptible to developing full-blown Alzheimer’s in later life are those who lose the most synapses, Dr. Thinakaran said.

Among the Cell Reports study highlights:

  • Loss of BIN1 expression in neurons leads to impaired spatial learning and memory. That is, the deficit alters how effectively information about surrounding environmental space is acquired, stored, organized and used. The BIN1 knockout mice had significantly more difficulty than controls in finding the hidden platform in a Morris water maze.
  • Further analysis found that BIN1 is primarily located on neurons that send neurotransmitters across the synapse (presynaptic sites) rather than residing on those neurons that receive the neurotransmitter messages (postsynaptic sites). Synaptic transmission in the hippocampus, a brain region associated primarily with memory, showed deterioration in the release of neurotransmitters from vesicles. Vesicles are bubble-like carriers that transfer neurotransmitters from presynaptic to postsynaptic neurons.
  • The BIN1 deficiency was associated with reduced density of synapses and a decrease in the number of synaptic clusters in the knockout mice compared to controls.
  • 3-D electron microscopy reconstruction of the synapses showed a significant accumulation of docked and reserve pools of synaptic vesicles in the BIN1 knockout mice. That indicates slower (less successful) release of neurotransmitters from their vesicles, the researchers suggest.

Super-resolution imaging of BIN1 (green) combined with pre- and postsynaptic sites. White arrows at right indicate the overlap between BIN1 and synapsin, a protein involved in regulating neurotransmitter release at synapses. | Image courtesy of Gopal Thinakaran, USF Health Morsani College of Medicine, appeared first in Cell Reports (supplementary materials) doi.org/10.1016/j.celrep.2020.02.026

The study authors conclude that altogether their work highlights a non-redundant role for neuronal BIN1 in presynaptic regulation and “opens new paths for the future investigation of the precise role of BIN1 as a risk factor in Alzheimer’s disease pathophysiology.”

The research was supported by grants from the National Institutes of Health, the Cure Alzheimer’s Fund, and the Alzheimer’s Association, as well as fellowships from the BrightFocus Foundation and the Illinois Department of Public Health.

 



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Gopal Thinakaran pursues genetic clues to Alzheimer’s disease pathways https://hscweb3.hsc.usf.edu/blog/2020/02/04/gopal-thinakaran-pursues-genetic-clues-to-alzheimers-disease-pathways/ Tue, 04 Feb 2020 19:44:18 +0000 https://hscweb3.hsc.usf.edu/?p=30657 The USF Health neurobiologist focuses on understanding genetic risk factors that may offer new therapy targets to delay or protect against age-related cognitive decline There has been a […]

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The USF Health neurobiologist focuses on understanding genetic risk factors that may offer new therapy targets to delay or protect against age-related cognitive decline

There has been a steep rise in the number of Americans dying of Alzheimer’s disease – up 145 percent between 2000 and 2017  The burden of this neurodegenerative disease, which relentlessly diminishes the mind, is not only borne by those living more years in a state of disability and dependence before dying, but by the family members who care for them.

No treatments exist to cure or slow the progression of Alzheimer’ disease, the major form of dementia afflicting an estimated 5.8 million Americans.

“The goal of our research is to reduce the (brain) pathology leading to Alzheimer’s disease, by identifying targeted treatments to delay the onset of disease and protect cognitive function,” said Gopal Thinakaran, PhD, professor of molecular medicine and associate dean for neuroscience research at the USF Health Morsani College of Medicine. “Finding ways to extend cognitive function so that an older person is still able to continue their daily activities or recognize a loved one – even for five more years – would greatly benefit both those suffering from Alzheimer’s and their families or other caregivers.”

Gopal Thinakaran, PhD (center), who holds the Bagnor Endowed Chair in Alzheimer’s Research, with his research team at the USF Health Byrd Alzheimer’s Center.

Dr. Thinakaran, an internationally recognized Alzheimer’s disease researcher, joined the University of South Florida from the University of Chicago in August to help accelerate the interdisciplinary work of the USF Health Neuroscience Institute. That includes recruiting a critical mass of basic scientists who can complement the university’s ongoing Alzheimer’s research while also expanding efforts to translate laboratory findings into new therapies for other neurodegenerative disorders, including Parkinson’s disease, ataxias, ALS, and multiple sclerosis.

Probing molecular, cellular changes underlying pathology

In addition to his leadership role, Dr. Thinakaran oversees a laboratory at the Byrd Alzheimer’s Center where he uses cutting-edge cell biology techniques and mouse models to study the molecular and cellular processes underlying Alzheimer’s disease. His research is supported by more than $6.1 million in grants from the National Institutes of Health (NIH), National Institute on Aging.

With normal brain aging, people experience minor lapses of memory (i.e., forgetting where their keys were left, or the name of someone just met) and some reduced speed in processing information. But disruptions in attention, memory, language, thinking and decision-making that interfere with daily life are signs of dementia.

In addition to overseeing his own laboratory research, Dr. Thinakaran holds Morsani College of Medicine leadership roles as associate dean of neuroscience research and Neuroscience Research Institute associate director of research.

Dr. Thinakaran’s lab pursues findings on relatively new genes identified through genome-wide association studies to gain insights into the mechanisms of late-onset Alzheimer’s disease, which affects people age 65 or older and accounts for the overwhelming majority of cases. Recently, the group has been investigating the role of bridging integrator 1 (BIN1), the second most common genetic risk factor for late-onset Alzheimer’s (exceeded only by APOE). Approximately 40% of people with Alzheimer’s have one of three variations in the BIN1 gene – a glitch in a single DNA building block (nucleotide) that heightens their risk for the disease, Dr. Thinakaran said.

Pursuing a common risk factor for late-onset Alzheimer’s

BIN1, expressed in all the body’s cells, has been shown to play a role in suppressing tumors and in muscle development — but little is known about what the protein does in the brain. Dr. Thinakaran was among the first to embrace the challenge of pursuing how BIN1 contributes to Alzheimer’s disease risk at a time when most researchers focused on amyloid and tau, two proteins considered the primary drivers of Alzheimer’s pathology.

Now, his team and a few others across the country probe what goes wrong in Alzheimer’s patients who carry the BIN1 risk allele. They have already confirmed that BIN1 is present both in the brain’s nerve cells (neurons) and its non-neuronal cells, such as oligodendrocytes and microglia.

Biochemist Melike Yuksel, PhD, a postdoctoral scholar in Dr. Thinakaran’s lab

A healthy human brain contains tens of billions of neurons that process and transmit chemical messages (neurotransmitters) across a tiny gap between neurons called a synapse. Alzheimer’s disease severely disrupts this synaptic communication, eventually killing cells throughout the brain and leading to a steep decline in memory and other signs of dementia.

“The single biggest correlation with cognitive decline is the loss of these synaptic communication centers between neurons,” Dr. Thinakaran said, adding that individuals most susceptible to developing full-blown Alzheimer’s in later life are those who lose the most synapses.

In a study published March 10 in Cell Reports, Dr. Thinakaran and colleagues demonstrated for the first time that the loss of BIN1 expression impaired spatial learning and memory associated with remembering where things are located. The researchers used an Alzheimer’s disease “knockout” mouse model in which neuronal BIN1 expression was inactivated in the hippocampus, a brain region involved with higher cognitive functions.

Discovering a defect in brain cell communication

A lack of BIN1 leads to a defect in the transmission of neurotransmitters needed to activate the brain cell communication that allows us to think and behave, the researchers found. Further analysis found that BIN1 was primarily located in neurons that send neurotransmitters across the synapse (presynaptic sites) rather than residing on those neurons that receive the neurotransmitter messages (postsynaptic sites). The BIN1 deficiency was also associated with reduced synapse density; a back-up of docked vesicles, the tiny bubble-like carriers that transfer neurotransmitters from presynaptic to postsynaptic neurons; and likely slower release of the neurotransmitters from their vesicles.

“Our findings so far that BIN1 localizes right at the point of (presynaptic) communication and may be precisely regulating neurotransmitter vesicle release brings us much closer to understanding how BIN1 could exert its function as a risk factor (for Alzheimer’s disease),” Dr. Thinakaran said. “We suspect it helps control how efficiently neurons communicate.”

Peering into the brain, one synapse at a time. Electron micrograph depicting selected region of a mouse brain hippocampus, the brain area responsible for learning and memory. A single synapse is marked with the yellow outline.  The human brain is estimated to have trillions of these synapses, which transmit information from one neuron to the next.| Image courtesy of Gopal Thinakaran, PhD

Antibody-stained mouse brain with Alzheimer’s disease β-amyloid deposits. The amyloid precursor proteins within healthy nerve cells and swollen neuronal processes are depicted in blue. The late-onset Alzheimer’s risk factor BIN1 is shown in green, and a marker for brain glial cells responsible for neuroinflammation is shown in magenta.| Image courtesy of Gopal Thinakaran, PhD

Dr. Thinakaran’s team also became interested in investigating whether BIN1 risk variants can interfere with the protective capacity of glia (cells supporting neurons) to mount a full inflammatory response needed to clear toxins from the brain. His USF Health group will work with researchers at Emory University to further investigate why the absence of BIN1 may impair the brain’s removal of abnormal beta-amyloid protein associated with Alzheimer’s disease.

Exploring the type 2 diabetes connection

Collaborating with a coprincipal investigator at the University of Kentucky, Dr. Thinakaran also explores the molecular link between type 2 diabetes and Alzheimer’s disease progression. An Alzheimer’s mouse model created by the Thinakaran lab allows researchers to turn on, or switch off, production of the human hormone amylin in the pancreas.

Amylin is secreted by the pancreas at higher levels, along with insulin, as diabetes begins to develop. Small amounts of this excess amylin migrate from pancreatic cells into the bloodstream and can cross the blood-brain barrier, especially in older brains where the protective barrier becomes leakier. The amylin then mixes with the brain’s beta-amyloid, which eventually builds into the sticky amyloid plaques that are a hallmark of Alzheimer’s pathology. The researchers will test in their preclinical model whether this brain amylin elevates the risk for Alzheimer’s disease, and if reducing amylin in peripheral circulation can help prevent or slow damage to cognition.

Dr. Thinakaran with biological scientist Stanislau (Stas) Smirnou

Scientists are still trying to figure out why some people remain cognitively resilient throughout life despite having neuropathology that would otherwise cause dementia. On the horizon, Dr. Thinakaran said, integrating large databases of gene expression and individual cell types will help scientists drill deeper into what specific inflammatory, metabolic and neural circuit changes shift a normally aging brain to one in which the abilities to remember, think and reason abnormally accelerate.

At the same time, data on genetics and environment/lifestyle (including diet, physical and mental exercises, sleep patterns and uncontrolled cardiovascular risk factors such as hypertension, diabetes and high cholesterol) are being collected both for patients in various stages of Alzheimer’s disease and for older adults with healthy cognitive function. “Bridging these two sets of data will be extremely valuable in understanding what confers higher risk and delineating what can keep our brains healthy as we age,” Dr. Thinakaran said.

Fascinated by a field with unprecedented challenges

Dr. Thinakaran holds a PhD in molecular biology and genetics from the University of Guelph in Canada. He completed a postdoctoral research fellowship in neuropathology and was an assistant professor of pathology at Johns Hopkins University School of Medicine. Before joining USF Health, he was a professor of neurobiology at the University of Chicago, where he built one of the country’s leading laboratories investigating pathways responsible for Alzheimer’s disease pathology and neuronal dysfunction.

Known as an accomplished scientist and thought leader who does not hesitate to tackle uncharted territory, Dr. Thinakaran studied muscle differentiation as a PhD student. But, he soon realized that muscle research had advanced to a stage where it was unlikely he could make much of an impact. At that time (early 1990s) Alzheimer’s disease research was just gaining momentum in molecular and cellular biology and posing unprecedented challenges, he said.

Biological scientist Xiaolin Zhang, MS

Once Dr. Thinakaran’s interest in Alzheimer’s was sparked during his postdoctoral training at Johns Hopkins, he seized the opportunity to pursue the emerging area of neuroscience research. “In many ways the brain and its complexity as we age is the final frontier in understanding human behavior. We’re continuing to learn every day the basics of how this organ system works, and what goes wrong when it doesn’t,” he said. “It’s a field that still has great opportunities for the next generation of young minds to make a difference.”

Dr. Thinakaran has authored more than 140 peer-reviewed publications. He is associate editor for the journals Molecular Neurodegeneration and Genes and Diseases and an editorial board member for Neurodegenerative Diseases and for Current Alzheimer Research. He serves on several scientific review/advisory committees for federal, private and public institutions. Dr. Thinakaran has received numerous awards, including the Alzheimer’s Association prestigious Zenith Fellows Award supporting senior scientists pursuing new ideas to advance Alzheimer’s and dementia research.

Some things you may not know about Dr. Thinakaran
  • Dr. Thinakaran combines his artistic talents of drawing and painting with his research. Andy Warhol-like microscopic art he created won a competition and was featured as the program cover for a brain research symposium at the University of Chicago. The multicolor montage of images depicts a mouse brain section (hippocampus) stained to visualize β-secretase, an enzyme critical for generating the hallmark Alzheimer’s disease β-amyloid pathology.
  • He is married to neurophysiologist Angèle Parent, PhD, associate professor of molecular medicine at the Byrd Alzheimer’s Center. They have three children: Abigaël, a freshman and aspiring neuroscientist at the University of Chicago; Daphné, 14; and Cédric, 12.
  • Dr. Thinakaran enjoys cooking authentic South Indian food and other international dishes with his family.

This microscopic brain art created by Dr. Thinakaran was featured on the program cover of a University of Chicago brain research symposium.

-Video by Allison Long, and photos by Freddie Coleman, USF Health Communications and Marketing



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USF PhD graduate in neuroscience to join laboratory of new Nobel Prize winner https://hscweb3.hsc.usf.edu/blog/2013/10/17/usf-phd-graduate-in-neuroscience-to-join-laboratory-of-new-nobel-prize-winner/ Thu, 17 Oct 2013 12:47:56 +0000 https://hscweb3.hsc.usf.edu/?p=9307 USF Health PhD graduate Justin Trotter leaves this weekend for Stanford University School of Medicine in Palo Alto, CA, where he will work for a recent Nobel Prize […]

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USF Health PhD graduate Justin Trotter leaves this weekend for Stanford University School of Medicine in Palo Alto, CA, where he will work for a recent Nobel Prize winner in medicine.  Trotter’s postdoctoral fellowship will play out in the large laboratory of Stanford neuroscience researcher Thomas Sudhof, MD, a Howard Hughes Medical Institute investigator who just last week jointly won the 2013 Nobel Prize in Physiology or Medicine for insights into the traffic control system for living cells.

Justin Trotter_Edwin Weeber_600x400

Justin Trotter, PhD, (left) USF Health neuroscience graduate, with major professor Edwin Weeber, PhD, chief scientific officer of the USF Health Byrd Alzheimer’s Institute.

“That just doesn’t happen very often,” said Edwin Weeber, PhD, professor of molecular pharmacology and physiology and chief scientific officer of the USF Health Byrd Alzheimer’s Institute.   Dr. Weeber was Trotter’s major professor while the young neuroscientist completed his doctoral studies in neuroscience at the Byrd Institute.

“Justin is the most driven graduate student I’ve had the pleasure to mentor,” Dr. Weeber said. “His ability to gain a fellowship with a Nobel Prize winner in one of the country’s top laboratories shows that USF and the Byrd Institute are training the next generation of scientists whose research will make a real difference.”

Trotter successfully defended his doctoral dissertation “Cellular and Molecular Mechanisms of Reelin Signaling in the Adult Hippocampus” on Sept. 27.  His doctoral research focused on signaling pathways important in brain development and their role in molecular mechanisms that give rise to learning and memory and that may be disrupted by Alzheimer’s disease.

“When I spoke to Tom (Sudhof) to endorse Justin’s application to his laboratory, I told him that Justin was the most brilliant young scientist I had ever met,” said Joachim Herz, MD, of the Department of Molecular Genetics, University of Texas Southwestern Medical Center, who served as the external chair for Trotter’s dissertation committee.

“My only concern and advice to Tom was that he should cut his travels short in the future, otherwise he might find Justin running the laboratory upon his return.”

Tom Sudhof

2013 Nobel Prize-winning neuroscience researcher Thomas Sudof, MD

Probing the neurobiology of learning and memory

For a person to think, move, feel or remember, the neurons in that person’s brain must communicate across junctions known as synapses. Increasing evidence has linked impairments in synaptic transmission to diseases such as Alzheimer’s and autism.

Working with Dr. Sudhof’s team, Trotter will still study how nerve cells communicate with one another to precisely exchange information across synapses within millisecond timescales. But at Stanford he will focus on the role of signaling pathways in autism instead of Alzheimer’s.

Trotter was offered postdoctoral fellowships at three of the country’s leading research institutions — the National Institutes of Health Eunice Kennedy Schriver National Institute of Child Health and Human Development, the Gladstone Institute affiliated with the University of California San Francisco, and, his first choice, Stanford University

He interviewed with Dr. Sudhof and 15 of his postdoctoral fellows July 19 and was offered the position by Dr. Sudhof  himself a week later, well before the Nobel Prize announcement.

“When I saw that he was on a team that made the finalists, I thought wouldn’t it be fun if he actually won,” Trotter said. “Then, the awards committee made the announcement the next day, and people were congratulating me – but I didn’t really do anything.”

Early fascination with science cultivated on a fish farm

Trotter grew up in Palm Bay, FL, but spent most weekends working on a tropical fish farm in nearby Fellsmere, where his father and grandfather operated the acquaculture business. He attributes his early fascination with science to what he learned on the fish farm, including how to breed and care for African cichlids.

In elementary school, while many classmates relied on their parents for help with science fair projects, Trotter looked forward to the challenge of creating and carrying out his own experiments.

“Science projects became my means of self-expression,” said Trotter, who won many regional and state science fair awards throughout middle and high school.  “I enjoyed designing experiments to test assumptions and garner facts about the natural world.”

By 10th grade he worked his way into an independent research project at Florida Institute of Technology, where he studied the molecular biology of starfish fertilization.  During one late-night experiment, Trotter said, he accidently hit his hand on a glass pipette filled with mercury. A hospital X-ray showed the shattered glass (from the broken pipette) scattered around a joint where the mercury injected.

“After about a week my hand was swollen and I had to get it operated on,” he said. “To this day I still have a small black, metallic circle near the injection site… Fortunately the type and quantity of mercury I was exposed to poses no danger.”

By 11th grade Trotter was testing algae extracts from the Indian River Lagoon and the Antarctica for anti-cancer properties.  He even set up a temporary lab culture room at his house when the laboratory space where he worked was taken over by scientists preparing experiments to accompany a Columbia space shuttle flight.

“I needed to determine whether the extracts that I had prepared possessed the ability to slow down the division rate of leukemia cells,” he said. “Needless to say, my mom learned to avoid wondering what I was doing in the den.”

Justin Trotter_600x400

Passion for neuroscience nurtured at USF

Trotter has spent the last five years at USF, where he says his passion for neuroscience was nurtured. He earned a bachelor of science degree in biomedical sciences here, followed by a master’s of science and PhD degrees in medical sciences, both with a concentration in neuroscience.

Along the way he has co-authored 15 journal articles with Dr. Weeber and/or other USF faculty members, including Lynn Martin, PhD, of the Department of Integrative Biology.  He was a member of an interdisciplinary team awarded a highly competitive 2012-13 USF Graduate Student Research Challenge Grant.  Dr. Weeber and Trotter also have a patent pending for a new therapeutic approach for treating brain injuries.

At the USF Health Byrd Alzheimer’s Institute, Trotter found in Dr. Weeber a mentor who shared his passion for delving into how synapses work with the hope that the research will lead to future treatments for Alzheimer’s disease or other cognitive disorders.

“I’ve really enjoyed the collaborative spirit at the Byrd Institute,” Trotter said.  “They’ve provided me with the resources and support needed to move forward in the development of my scientific career.”

And this USF graduate continues to move ahead — taking his place next month in the laboratory of a new Nobel Prize winner.  “It’s an amazing opportunity,” Trotter said.

– Photos by Eric Younghans, USF Health Communications

 

 



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